Active Recombinant Human CD3D Protein (Phe22-Ala105), C-Fc-tagged

• Cat.No.: CD3D-28H
• Product Overview: Recombinant human CD3 delta/CD3d protein (Phe22-Ala105) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Phe22-Ala105
• Description: The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.
• Form: Disulfide linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 delta/CD3d Fc Chimera is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Recombinant Human CD3E Fc Chimera Biotinylated Protein that produces 50% of the optimal binding response is approximately 1.0-6.0 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 36 kDa
  SDS-PAGE: 45-55 kDa, under reducing conditions
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CD3D CD3d molecule, delta (CD3-TCR complex) [ Homo sapiens (human) ]
• Official Symbol: CD3D
• Synonyms: CD3D; CD3d molecule, delta (CD3-TCR complex); CD3d antigen, delta polypeptide (TiT3 complex) , T3D; T-cell surface glycoprotein CD3 delta chain; CD3 delta; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex); T3D; CD3-DELTA
• Gene ID: 915
• mRNA Refseq: NM_000732
• Protein Refseq: NP_000723
• MIM: 186790
• UniProt ID: P04234