Active Recombinant Human CD47 Protein, LIgG2b Fc-tagged, low endotoxin

• Cat.No.: CD47-347H
• Product Overview: Recombinant Human CD47 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3). Predicted N-terminus: Gln 19. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: lIgG2b
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human SIRP alpha, His Tag at 0.5 μg/mL (100 μL/well) can bind Recombinant Human CD47 Protein, LIgG2b Fc-tagged, low endotoxin with a linear range of 0.1-8 ng/mL (QC tested).
• Molecular Mass: 41.6 kDa
• Protein length: Gln 19 - Pro 139
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD47 CD47 molecule [ Homo sapiens ]
• Official Symbol: CD47
• Synonyms: CD47; CD47 molecule; CD47 glycoprotein; IAP; OA3; Rh related antigen; Rh-related antigen; MER6
• Gene ID: 961
• mRNA Refseq: NM_001777
• Protein Refseq: NP_001768
• MIM: 601028
• UniProt ID: Q08722