Active Recombinant Human CD70 protein, His-Avi-Flag-tagged, Biotinylated

• Cat.No.: CD70-2986H
• Product Overview: Biotinylated Recombinant Human CD70 protein(Ser 52-Pro 193), fused with His-Avi-Flag tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi-Flag
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD27, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag with a linear range of 0.1-4 ng/mL.Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27, Fc Tag: Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag binding reactions. The half maximal inhibitory concentration (IC50) is 3.05858 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™) and a flag tag.The protein has a calculated MW of 51.0 kDa. The protein migrates as 47-57 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Ser 52-Pro 193
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD70 CD70 molecule [ Homo sapiens ]
• Official Symbol: CD70
• Synonyms: CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD27LG; TNFSF7
• Gene ID: 970
• mRNA Refseq: NM_001252
• Protein Refseq: NP_001243
• MIM: 602840
• UniProt ID: P32970