Source : |
HEK293 |
Species : |
Human |
Tag : |
His-Flag |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Anti-CD27 Ligand Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD27 Ligand, His,Flag Tag with a linear range of 0.039-0.156 μg/mL.Immobilized Human CD27, Fc Tag at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human CD27 Ligand, His,Flag Tag with a linear range of 0.039-0.156 μg/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.The protein has a calculated MW of 49.3 kDa. The protein migrates as 50-55 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Ser 52-Pro 193 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |