Active Recombinant Human CD70 protein, lFc-tagged

• Cat.No.: CD70-328H
• Product Overview: Recombinant Human CD70 protein(Ser 52 - Pro 193), fused with lFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: lFc
• Protein Length: Ser 52 - Pro 193
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human CD27 Ligand, Llama IgG2b Fc Tag at 2 μg/mL (100 μL/well) can bind Human CD27, His Tag with a linear range of 4-63 ng/mL.Immobilized Human CD27 Ligand, Llama IgG2b Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27, Fc,Avitag with a linear range of 0.3-20 ng/mL.
• Molecular Mass: This protein carries a llama IgG2b Fc tag at the N-terminusThe protein has a calculated MW of 75.5 kDa. The protein migrates as 140-160 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD70 CD70 molecule [ Homo sapiens ]
• Official Symbol: CD70
• Synonyms: CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD27LG; TNFSF7
• Gene ID: 970
• mRNA Refseq: NM_001252
• Protein Refseq: NP_001243
• MIM: 602840
• UniProt ID: P32970