| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
lFc |
| Protein Length : |
Ser 52 - Pro 193 |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human CD27 Ligand, Llama IgG2b Fc Tag at 2 μg/mL (100 μL/well) can bind Human CD27, His Tag with a linear range of 4-63 ng/mL.Immobilized Human CD27 Ligand, Llama IgG2b Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27, Fc,Avitag with a linear range of 0.3-20 ng/mL. |
| Molecular Mass : |
This protein carries a llama IgG2b Fc tag at the N-terminusThe protein has a calculated MW of 75.5 kDa. The protein migrates as 140-160 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 0.01 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
