Active Recombinant Human CD80 Protein, Fc & Avi-tagged, Biotinylated

Cat.No. : CD80-583H
Product Overview : Biotinylated Recombinant Human CD80 Protein (Val 35 - Asn 242) (NP_005182.1), fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : Fc&Avi
Predicted N Terminal : Val 35
Form : Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4, Fc Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human B7-1, Fc Tag with a linear range of 0.6-9.8 ng/mL.
Molecular Mass : Recombinant Human CD80 Protein has a calculated MW of 52.3 kDa. As a result of glycosylation, The protein migrates as 65-95 kDa under reducing (R) condition and 130-160 kDa under non-reducing (NR) condition on SDS-PAGE gel.
Protein length : Val 35 - Asn 242
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : CD80 CD80 molecule [ Homo sapiens ]
Official Symbol : CD80
Synonyms : CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) , CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1;
Gene ID : 941
mRNA Refseq : NM_005191
Protein Refseq : NP_005182
MIM : 112203
UniProt ID : P33681
Chromosome Location : 3q13.3-q21
Pathway : Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem;
Function : coreceptor activity; protein binding; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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