Active Recombinant Human CD80 Protein, Flag&His tagged

• Cat.No.: CD80-60H
• Product Overview: CD80 dimer protein contains a CD80 extracellular domain (UniProt# P33681) fused with a proprietary dimer motif followed by a tandem Flag-His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293T
• Tag: Flag&His
• Protein Length: 135-242 aa
• Description: Human CD80 (Cluster of differentiation 80) is a type I transmembrane glycoprotein in the immunoglobulin superfamily and is a member of the B7 Family of ligands. CD80 is also known as B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, and LAB7. CD80 contains an extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain. The ECD consists of two immunoglobulin (Ig)-like subdomains, a variable-like domain (Ig-V-like domain), and a constant-like domain (Ig-C-like domain). It is primarily expressed on antigen-presenting cells (APCs), such as dendritic cells, macrophages, and B cells. CD80 interacts with CTLA-4 (Cytotoxic T-lymphocyte associated protein 4) to transmit an inhibitory signal with T cells and with CD28 (Cluster of differentiation 28) to transmit a stimulatory signal. It is often overexpressed in various autoimmune diseases such as multiple sclerosis and systemic lupus erythematosus, as well as some cancers. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. A recombinant protein mimicking the CD80 dimer conformation can be crucial for therapeutic discovery.
• Molecular Mass: 66 kDa
• Homodimer/Heterodimer: Homodimer
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA for CD80-specific antibody and CTLA-4 or CD28 ligand protein binding assays. Suggested Applications ELISA for CD80-specific antibody and CTLA-4 or CD28 ligand protein binding assays. SPR & BLI for CD80-specific antibody: and CTLA-4 or CD28 protein binding assays. Animal immunization, RUO.
• Storage: At -80 centigrade
• Storage Buffer: 0.22μm filtered PBS, pH 7.4
• Shipping: Frozen Dry Ice

Gene Information

• Gene Name 2: CD80 CD80 molecule [Homo sapiens (human)]
• Official Symbol 2: CD80
• Synonyms 2: CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen), CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1
• Gene ID 2: 941
• mRNA Refseq 2: NM_005191
• Protein Refseq 2: NP_005182
• MIM 2: 112203
• UniProt ID 2: P33681