| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Fc |
| Protein Length : |
Leu 26-Pro 247 |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human B7-2, Fc Tag, premium grade at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag with a linear range of 0.6-10 ng/mL.Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human B7-2, Fc Tag, premium grade with a linear range of 5-78 ng/mL.Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human B7-2, Fc Tag, premium grade with a linear range of 2-78 ng/mL. |
| Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 51.5 kDa. The protein migrates as 66-100 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 0.01 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
