Active Recombinant Human CD8A&CD8B Heterodimer protein, His-Flag-tagged, Low Endotoxin

• Cat.No.: CD8A & CD8B-211H
• Product Overview: Recombinant Human CD8A&CD8B Heterodimer protein(P01732-1(CD8 alpha) & P10966-1(CD8 beta))(22-182 aa(CD8A) & 22-170 aa(CD8B)), fused with C-terminal His and Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 22-182 aa(CD8A) & 22-170 aa(CD8B)
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD8A&CD8B, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11. 3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 32-38 kDa and 40-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD8A CD8a molecule [ Homo sapiens ]
• Official Symbol: CD8A
• Synonyms: CD8A; CD8a molecule; CD8, CD8 antigen, alpha polypeptide (p32) , T cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; p32; Leu2
• Gene ID: 925
• mRNA Refseq: NM_001145873
• Protein Refseq: NP_001139345
• MIM: 186910
• UniProt ID: P01732
• Gene Name 2: CD8B CD8b molecule [ Homo sapiens ]
• Official Symbol 2: CD8B
• Synonyms 2: CD8B; CD8b molecule; CD8 antigen, beta polypeptide 1 (p37) , CD8B1; T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain; LY3; P37; LEU2; LYT3; CD8B1; MGC119115
• Gene ID 2: 926
• mRNA Refseq 2: NM_001178100
• Protein Refseq 2: NP_001171571
• MIM 2: 186730
• UniProt ID 2: P10966