Active Recombinant Human CDK4/CCND1 Protein, GST-tagged

• Cat.No.: CDK4/CCND1-274H
• Product Overview: Recombinant full-length human CDK4 and CyclinD1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 12nmol/min/mg
• Molecular Mass: ~57 kDa and CyclinD1 ~61 kDa
• Purity: >75%
• Storage: Storage at -70 centigrade
• Concentration: 0.1ug/ul

Gene Information

• Gene Name: CDK4 cyclin-dependent kinase 4 [ Homo sapiens ]
• Official Symbol: CDK4
• Synonyms: CDK4; cyclin-dependent kinase 4; PSK J3; cell division protein kinase 4; CMM3; PSK-J3; MGC14458
• Gene ID: 1019
• mRNA Refseq: NM_000075
• Protein Refseq: NP_000066
• MIM: 123829
• UniProt ID: P11802