Active Recombinant Human CDK4 Protein, His-Tagged

• Cat.No.: CDK4-1016H
• Product Overview: Human CDK4 (NM_000075, 4 a.a. - 303 a.a.) and CCND3 (NM_001760, 1 a.a. - 292 a.a.) recombinanat protein with His His tag expressed in Sf9 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 4-303;1-292 a.a.
• Description: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
• Form: Liquid
• Bio-activity: 75 pmol/μg x min
• Molecular Mass: CDK4: 59.700, CCND3 kDa
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing
• Concentration: 0.337 μg/μL
• Storage Buffer: In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)

Gene Information

• Gene Name: CDK4 cyclin-dependent kinase 4 [ Homo sapiens ]
• Official Symbol: CDK4
• Synonyms: CDK4; cyclin-dependent kinase 4; PSK J3; cell division protein kinase 4; CMM3; PSK-J3; MGC14458
• Gene ID: 1019
• mRNA Refseq: NM_000075
• Protein Refseq: NP_000066
• MIM: 123829
• UniProt ID: P11802