Active Recombinant Human CDKN2D protein, GST-tagged

• Cat.No.: CDKN2D-2502H
• Product Overview: Active Recombinant Human CDKN2D protein(P55273) (Met 10-Leu 166) was expressed in E.coli, fused with the GST tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 10-166 aa
• Form: Lyophilized from sterile PBS, pH 7.5.
• Bio-activity: Immobilized human GST-CDKN2D at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 0.52-1.2 μg/ml.
• Molecular Mass: The recombinant human CDKN2D/GST chimera consists of 400 amino acids and has a predicted molecular mass of 44.9 kDa. It migrates as an approxiamtely 46 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CDKN2D cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) [ Homo sapiens ]
• Official Symbol: CDKN2D
• Synonyms: CDKN2D; cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4); cyclin-dependent kinase 4 inhibitor D; INK4D; p19; CDK inhibitor p19INK4d; inhibitor of cyclin-dependent kinase 4d; cyclin-dependent kinase 4 inhibitor D p19; cell cycle inhibitor, Nur77 associating protein; p19-INK4D
• Gene ID: 1032
• mRNA Refseq: NM_001800
• Protein Refseq: NP_001791
• MIM: 600927
• UniProt ID: P55273