Active Recombinant Human CHEK1 Protein, GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CHEK1-229HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.

Specification

• Source: Insect cells
• Species: Human
• Tag: GST
• Bio-activity: The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
• Molecular Mass: The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7 kDa. It migrates as an approxiamtely 80 kDa band as predicted in SDS-PAGE under reducing conditions.
• Protein length: 700
• N-terminal Sequence Analysis: Met
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 2mM GSH.

Gene Information

• Gene Name: CHEK1 checkpoint kinase 1 [ Homo sapiens ]
• Official Symbol: CHEK1
• Synonyms: CHK1; CHK1 checkpoint homolog; Checkpoint, S. pombe, homolog of, 1; Chk1-S; cell cycle checkpoint kinase; checkpoint kinase-1
• Gene ID: 1111
• mRNA Refseq: NM_001114121
• Protein Refseq: NP_001107593
• MIM: 603078
• UniProt ID: O14757