Active Recombinant Human CHEK1 Protein, GST-tagged

• Cat.No.: CHEK1-229H
• Product Overview: Active Recombinant Human CHEK1 Protein(AAM78553.1) (Met1-Thr476) was expressed in Insect Cells, fused with the GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-476 aa
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM GSH.
• Bio-activity: The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
• Molecular Mass: The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7kDa. It migrates as an approxiamtely 80 KDa band as predicted in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CHEK1 checkpoint kinase 1 [ Homo sapiens ]
• Official Symbol: CHEK1
• Synonyms: CHEK1; checkpoint kinase 1; CHK1 (checkpoint, S.pombe) homolog , CHK1 checkpoint homolog (S. pombe); serine/threonine-protein kinase Chk1; CHK1; Chk1-S; checkpoint kinase-1; CHK1 checkpoint homolog; cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1
• Gene ID: 1111
• mRNA Refseq: NM_001114121
• Protein Refseq: NP_001107593
• MIM: 603078
• UniProt ID: O14757