Recombinant Human CHIT1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CHIT1-2701H
• Product Overview: CHIT1 MS Standard C13 and N15-labeled recombinant protein (NP_003456) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 51.5 kDa
• AA Sequence: MVRSVAWAGFMVLLMIPWGSAAKLV CYFTNWAQYRQGEARFLPKDLDPSL CTHLIYAFAGMTNHQLSTTEWNDET LYQEFNGLKKMNPKLKTLLAIGGWN FGTQKFTDMVATANNRQTFVNSAIR FLRKYSFDGLDLDWEYPGSQGSPAV DKERFTTLVQDLANAFQQEAQTSGK ERLLLSAAVPAGQTYVDAGYEVDKI AQNLDFVNLMAYDFHGSWEKVTGHN SPLYKRQEESGAAASLNVDAAVQQW LQKGTPASKLILGMPTYGRSFTLAS SSDTRVGAPATGSGTPGPFTKEGGM LAYYEVCSWKGATKQRIQDQKVPYI FRDNQWVGFDDVESFKTKVSYLKQK GLGGAMVWALDLDDFAGFSCNQGRY PLIQTLRQELSLPYLPSGTPELEVP KPGQPSEPEHGPSPGQDTFCQGKAD GLYPNPRERSSFYSCAAGRLFQQSC PTGLVFSNSCKCCTWNTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CHIT1 chitinase 1 [ Homo sapiens (human) ]
• Official Symbol: CHIT1
• Synonyms: CHIT1; chitinase 1 (chitotriosidase); chitotriosidase-1; CHI3; CHIT; chitinase-1; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; CHITD; FLJ00314; MGC125322
• Gene ID: 1118
• mRNA Refseq: NM_003465
• Protein Refseq: NP_003456
• MIM: 600031
• UniProt ID: Q13231