Recombinant Human CHIT1 protein, His-tagged

• Cat.No.: CHIT1-535H
• Product Overview: Recombinant Human CHIT1 protein(NP_003456.1) (Met 1-Asn 466) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-466 aa
• Form: Lyophilized from sterile 12.5mM Tris, 75mM NaCl, 50% glycerol, pH 7.5.
• Molecular Mass: The recombinant human CHIT1 consists of 456 amino acids after removal of the signal peptide and has a predicted molecular mass of 50.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CHIT1 is approximately 48 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001243054.1
• Unit ID: Q13231
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/Q13231

Gene Information

• Gene Name: CHIT1 chitinase 1 (chitotriosidase) [ Homo sapiens ]
• Official Symbol: CHIT1
• Synonyms: CHIT1; chitinase 1 (chitotriosidase); chitotriosidase-1; CHI3; CHIT; chitinase-1; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; CHITD; FLJ00314; MGC125322
• Gene ID: 1118
• mRNA Refseq: NM_001256125
• protein Refseq: NP_001243054
• MIM: 600031