Active Recombinant Human CLEC5A/MDL-1 Protein, His-tagged, Active dimer

• Cat.No.: CLEC5A-11054H
• Product Overview: Human CLEC5A, His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 28 - Lys 188 (Accession # Q9NY25-1). This protein carries a polyhistidine tag at the N-terminus. Predicted N-terminus: His

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 28-188 aa
• Description: CLEC5A, also known as MDL1 and MDL-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily.CLEC5A is expressed on monocytes, macrophages, and neutrophils. Its expression is up-regulated on monocytes in rheumatoid arthritis. CLEC5A with dnax-activation protein 12 and may play a role in cell activation. It also functions as a positive regulator of osteoclastogenesis. CLEC5A acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: The purity of Human CLEC5A, His Tag is more than 95% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
• Molecular Mass: The protein has a calculated MW of 39.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CLEC5A C-type lectin domain family 5, member A [ Homo sapiens (human) ]
• Official Symbol: CLEC5A
• Synonyms: CLEC5A; C-type lectin domain family 5, member A; C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 5, CLECSF5; C-type lectin domain family 5 member A; MDL 1; C-type lectin superfamily member 5; myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 5; MDL1; MDL-1; CLECSF5; MGC138304
• Gene ID: 23601
• mRNA Refseq: NM_013252
• Protein Refseq: NP_037384
• MIM: 604987
• UniProt ID: Q9NY25