Active Recombinant Human CNTFR protein, His-tagged

• Cat.No.: CNTFR-3334H
• Product Overview: Active Recombinant Human CNTFR protein(Met 1- 346)(NP_001833.1) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-346 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 1.28-160 ng/ml.
• Molecular Mass: The recombinant human CNTFR consists of 335 amino acids and predicts a molecular mass of 36 kDa. The apparent molecular mass of rhCNTFR is apximately 45-48 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001193940.1
• Unit ID: P26992
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P26992

Gene Information

• Gene Name: CNTFR ciliary neurotrophic factor receptor [ Homo sapiens ]
• Official Symbol: CNTFR
• Synonyms: CNTFR; ciliary neurotrophic factor receptor; ciliary neurotrophic factor receptor subunit alpha; CNTFR-alpha; CNTF receptor subunit alpha; MGC1774
• Gene ID: 1271
• mRNA Refseq: NM_001207011
• protein Refseq: NP_001193940
• MIM: 118946