Active Recombinant Human CNTFR protein, His-tagged

• Cat.No.: CNTFR-3334H
• Product Overview: Recombinant Human CNTFR protein(NP_001833.1)(Met1-Pro346), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear range of 1.28-160 ng/ml.
• Molecular Mass: The recombinant human CNTFR consists of 335 amino acids and predicts a molecular mass of 36 kDa. The apparent molecular mass of rhCNTFR is approximately 45-48 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Pro346
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CNTFR ciliary neurotrophic factor receptor [ Homo sapiens ]
• Official Symbol: CNTFR
• Synonyms: CNTFR; ciliary neurotrophic factor receptor; ciliary neurotrophic factor receptor subunit alpha; CNTFR-alpha; CNTF receptor subunit alpha; MGC1774
• Gene ID: 1271
• mRNA Refseq: NM_001207011
• Protein Refseq: NP_001193940
• MIM: 118946
• UniProt ID: P26992