| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
Flag&His |
| Description : |
Background: Cereblon (CRBN) is the substrate recognition subunit of the CRL4-CRBN cullin E3 ubiquitin ligase complex. It facilitates the ubiquitylation and subsequent proteasomal degradation of proteins including MEIS2, ILF2 and others, and its activity is required for proper limb development. Binding of immunomodulatory drugs (IMiD' s) remodels CRBN' s surface, thereby changing its substrate binding preference. Substrates that are bound by CRBN only in the presence of IMiD' s are known as "neosubstrates." Expanding CRBN' s neo-substrate scope is a frontier for oncology, neurodegeneration and other research in the field of Targeted Protein Degradation (TPD). |
| Form : |
25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH 7.5. |
| Bio-activity : |
Verified in Ternary Complex Assay. |
| Molecular Mass : |
CRBN: 55 kDa
DDB1(ΔB): 96 kDa |
| AASequence : |
CRBN: MDYKDDDDKSAVDENLYFQGGGRGGSAHIVMVDAYKPTKGGSGMAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
DDB1(ΔB): MGSSHHHHHHHHSAVDENLYFQGGGRMSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGGNGNSGEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH |
| Purity : |
0.95 |
| Applications : |
CRBN / DDB1(ΔB) is active in ternary complex formation assays using recombinant BRD4 and the degrader molecule dBET-1, and is also useful in proteomics approaches. This protein will NOT interact with CUL4. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -70 centigrade (stable for 24 months from date of receipt). |
| Concentration : |
10-30 μM |
| Reference : |
1. Baek K., et al. (2025) Nature Comm 16(1): 6831 PMID: 40707481
2. Liu Y., Bai J., Li D., et al. (2025) Trends Biochem Sci. 50 (2): 134-142. PMID 39753433
3. Carter T.R., et al. (2024) Bioorg Med Chem. 104: 117699. PMID 38608634 |
