Active Recombinant Human CSF1R protein, Fc/Avi-tagged, Biotinylated
Cat.No. : | CSF1R-051H |
Product Overview : | Biotinylated Recombinant Human CSF1R(Ile20-Thr516) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
- Specification
- Gene Information
- Related Products
Description : | CSF1R is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10). |
Source : | CHO cells |
Species : | Human |
Tag : | Fc/Avi |
Predicted N Terminal : | Ile20 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human CSF1R is immobilized at 1 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human CSF1R Fc Chimera Avi-tag with an ED50 of 6-36 ng/mL. |
Molecular Mass : | 100-120 kDa, under reducing conditions |
Protein length : | Ile20-Thr516 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | CSF1R colony stimulating factor 1 receptor [ Homo sapiens ] |
Official Symbol : | CSF1R |
Synonyms : | CSF1R; colony stimulating factor 1 receptor; FMS, McDonough feline sarcoma viral (v fms) oncogene homolog; macrophage colony-stimulating factor 1 receptor; C FMS; CD115; CSFR; CSF-1-R; CD115 antigen; CSF-1 receptor; FMS proto-oncogene; proto-oncogene c-Fms; macrophage colony stimulating factor I receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; FMS; FIM2; HDLS; C-FMS; CSF-1R; M-CSF-R; |
Gene ID : | 1436 |
mRNA Refseq : | NM_005211 |
Protein Refseq : | NP_005202 |
MIM : | 164770 |
UniProt ID : | P07333 |
Products Types
◆ Lysates | ||
CSF1R-823RCL | Recombinant Rat CSF1R cell lysate | +Inquiry |
CSF1R-444HCL | Recombinant Human CSF1R cell lysate | +Inquiry |
CSF1R-2739HCL | Recombinant Human CSF1R cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1574 | CSF1R Kinase Binding Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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