Description : |
Epithelial cellderived neutrophilactivating peptide (ENA78) also known as C-X-C motif chemokine 5 (CXCL5), is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon, IFN-γ. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. Full length CXCL5 (78 aa) is trimmed at the Nterminal end by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70aa), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5can signal through the CXCR2 receptor. |
Source : |
HEK293T |
Species : |
Human |
Form : |
Lyophilized after extensive dialysis against PBS. |
Bio-activity : |
The EC50 value of human ENA-78/CXCL5 on Ca2+ mobilization assay in CHO-K1/ Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 200.0 ng/ml. |
Molecular Mass : |
~8.5 kDa |
Endotoxin : |
< 0.2 EU/μg of protein by gel clotting method. |
Purity : |
> 98% |
Storage : |
Upon receiving, this product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product should be stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |