Active Recombinant Human CXCL6 protein

• Cat.No.: CXCL6-171H
• Product Overview: Recombinant Human CXCL6 protein was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Description: Granulocyte chemotactic protein 2 (GCP-2) also known as Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family. As its former name suggests, GCP-2 is a chemoattractant for neutrophilic granulocytes. Among human CXC chemokines, GCP­2 is most closely related to ENA­78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP­2 and ENA­78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. GCP­2 can signal through the CXCR1 and CXCR2 receptors.
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: The EC50 value of human GCP-2/CXCL6 on Ca2+ mobilization assay in CHO-K1/ Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 0.8 μg/ml.
• Molecular Mass: ~9 kDa
• Endotoxin: < 0.2 EU/μg of protein by gel clotting method.
• Purity: > 98%
• Storage: Upon receiving, this product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product should be stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.

Gene Information

• Gene Name: CXCL6 C-X-C motif chemokine ligand 6 [ Homo sapiens (human) ]
• Official Symbol: CXCL6
• Synonyms: CXCL-6; SCYB6; GCP-2; CKA-3
• Gene ID: 6372
• mRNA Refseq: NM_002993.4
• Protein Refseq: NP_002984.1
• MIM: 138965
• UniProt ID: P80162