Active Recombinant Human CYP1A1 Protein
Cat.No. : | CYP1A1-04H |
Product Overview : | Recombinant Human CYP1A1 and human CYP-low reductase are coexpressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
Description : | This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. |
Source : | E.coli |
Species : | Human |
Form : | 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
Bio-activity : | Specific P450 Content:100 pmol/mg protein; Cytochrome c Reductase Activity:103 nmol/min/mg protein; P450 Activity:7-ethoxycoumarin O-deethylase; Linear with time up to:12 min; Linear with P450 up to:5 pmol/ml; Vmax:11 pmol/min/pmol CYP; Km:3.8 μM |
Notes : | 1. Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]; 2. Determined at 5 μM substrate, using a P450 concentration of 1 pmol/ml; 3. Determined at 5 μM substrate, using an incubation time of 5 min. |
Storage : | Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
Concentration : | 10 mg/ml |
Gene Name : | CYP1A1 |
Official Symbol : | CYP1A1 |
Synonyms : | AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX |
Gene ID : | 1543 |
mRNA Refseq : | NM_000499.5 |
Protein Refseq : | NP_000490.1 |
MIM : | 108330 |
UniProt ID : | P04798 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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