Active Recombinant Human DDB1/CRBN Complex Protein, His-tagged

• Cat.No.: DDB1-928H
• Product Overview: Active Recombinant Human DDB1/CRBN Complex Protein with N-terminal 6xHis tag was purified in Sf9 insect cell by NI-NTA affinity and followed by SEC chromatography. The CRBN/DDB1 protein showed high activity in HTRF assay.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: CRBN: M1-L442; DDB1: M1-H1140
• Tag: N-His
• Form: 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5.
• Bio-activity: The CRBN/DDB1 activity was detected using HTRF technology. The reaction was performed by incubating the varying concentration of lenalidomide, CRBN/DDB1 protein, substrate and beads at 25°C for 60 min, then reading ratio 665/620 with BMG.
• Molecular Mass: CRBN: 52.1 kDa; DDB1: 127.0 kDa
• Purity: > 95% by SDS-PAGE
• Storage: Stable for 12 months upon stored at -80°C from the date of receipt. And avoid repeated freeze-thaws cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• UniProt ID 1: Q16531
• GeneID 2: 51185

Gene Information

• Gene Name: DDB1 damage-specific DNA binding protein 1, 127kDa [ Homo sapiens ]
• Official Symbol: DDB1
• Synonyms: DDB1; damage-specific DNA binding protein 1, 127kDa; damage specific DNA binding protein 1 (127kD); DNA damage-binding protein 1; XPE; XAP-1; UV-DDB 1; DDB p127 subunit; XPE-binding factor; HBV X-associated protein 1; DNA damage-binding protein a; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; damage-specific DNA-binding protein 1; xeroderma pigmentosum group E-complementing protein; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
• Gene ID: 1642
• mRNA Refseq: NM_001923
• Protein Refseq: NP_001914
• MIM: 600045
• Gene Name 2: CRBN cereblon [ Homo sapiens ]
• Official Symbol 2: CRBN
• Synonyms 2: CRBN; cereblon; mental retardation, non syndromic, autosomal recessive, 2A , MRT2A; protein cereblon; protein x 0001; MRT2; MRT2A; MGC27358; DKFZp781K0715
• mRNA Refseq 2: NM_001173482
• Protein Refseq 2: NP_001166953
• MIM 2: 609262
• UniProt ID: Q96SW2