Active Recombinant Human DDR1 protein, Fc-tagged

• Cat.No.: DDR1-1942H
• Product Overview: Recombinant Human DDR1 protein(Asp 21 - Thr 416), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Human DDR1, Fc Tag on Protein A Biosensor, can bind Native Human Collagen I protein with an affinity constant of 0.327 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 70.4 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asp21-Thr416
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DDR1 discoidin domain receptor tyrosine kinase 1 [ Homo sapiens ]
• Official Symbol: DDR1
• Synonyms: DDR1; discoidin domain receptor tyrosine kinase 1; CAK, discoidin domain receptor family, member 1 , EDDR1, NEP, NTRK4, PTK3A; epithelial discoidin domain-containing receptor 1; CD167; RTK6; tyrosine kinase DDR; cell adhesion kinase; mammary carcinoma kinase 10; tyrosine-protein kinase CAK; protein-tyrosine kinase RTK-6; neuroepithelial tyrosine kinase; PTK3A protein tyrosine kinase 3A; CD167 antigen-like family member A; neurotrophic tyrosine kinase, receptor, type 4; CAK; DDR; NEP; HGK2; PTK3; TRKE; EDDR1; MCK10; NTRK4; PTK3A
• Gene ID: 780
• mRNA Refseq: NM_001202521
• Protein Refseq: NP_001189450
• MIM: 600408
• UniProt ID: Q08345