Active Recombinant Human DHODH protein, His-tagged

• Cat.No.: DHODH-11971H
• Product Overview: Recombinant Human DHODH protein(NP_001352.2)(Arg35~Asp392) was expressed in E.coli with a N-terminal His tag.

Specification

• Description: The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: The binding activity of DHODH with FKBP8.
• Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg35~Asp392
• AA Sequence: RFYAEHLMPTLQGLLDPESAHRLAV RFTSLGLLPRARFQDSDMLEVRVLG HKFRNPVGIAAGFDKHGEAVDGLYK MGFGFVEIGSVTPKPQEGNPRPRVF RLPEDQAVINRYGFNSHGLSVVEHR LRARQQKQAKLTEDGLPLGVNLGKN KTSVDAAEDYAEGVRVLGPLADYLV VNVSSPNTAGLRSLQGKAELRRLLT KVLQERDGLRRVHRPAVLVKIAPDL TSQDKEDIASVVKELGIDGLIVTNT TVSRPAGLQGALRSETGGLSGKPLR DLSTQTIREMYALTQGRVPIIGVGG VSSGQDALEKIRAGASLVQLYTALT FWGPPVVGKVKRELEALLKEQGFGG VTDAIGAD
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  (May be suitable for use in other assays to be determined by the end user.)
• Notes: The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8°C for one month.
  Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: DHODH dihydroorotate dehydrogenase (quinone)[Homo sapiens (human)]
• Official Symbol: DHODH
• Synonyms: DHOdehase; Dihydroorotate oxidase; Dihydroorotate dehydrogenase (quinone), mitochondrial
• Gene ID: 1723
• mRNA Refseq: NM_001361.5
• Protein Refseq: NP_001352.2
• MIM: 126064
• UniProt ID: Q02127