Active Recombinant Human DKK3 Protein, His-tagged

• Cat.No.: DKK3-001H
• Product Overview: Recombinant human DKK-3 (Ala22-Ile350) protein with a C-terminal 10-His tag was expressed in NS0 cells.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Tag: His
• Protein Length: 22-350 a.a.
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Hsieh, S.Y. et al. (2004) Oncogene 23:9183. The ED50 for this effect is 3-12 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 37.5 kDa
  SDS-PAGE: 75-80 kDa, reducing conditions
• N-terminal Sequence Analysis: Ala22
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 250 μg/mL in sterile PBS.

Gene Information

• Gene Name: DKK3
• Official Symbol: DKK3 dickkopf WNT signaling pathway inhibitor 3 [ Homo sapiens (human) ]
• Synonyms: dickkopf (Xenopus laevis) homolog 3; dickkopf 3; dickkopf homolog 3 (Xenopus laevis); Dickkopf-3; dickkopf-related protein 3; Dkk3; Dkk-3; hDkk-3; regulated in glioma; REIC; REICdkk-3; RIG; RIG-like 5-6; RIG-like 7-1; DKK3; dickkopf WNT signaling pathway inhibitor 3
• Gene ID: 27122
• mRNA Refseq: NM_001018057
• Protein Refseq: NP_001018067
• MIM: 605416
• UniProt ID: Q9UBP4