Active Recombinant Human DLL1 protein, His-tagged

• Cat.No.: DLL1-278H
• Product Overview: Active Recombinant Human DLL1 protein(NP_005609.3)(Met 1-Gly 540) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-540 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured by its ability to bind human NOTCH1 in a functional ELISA. 2. Measured by the ability of the immobilized protein to enhance BMP2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL.
• Molecular Mass: The recombinant human DLL1 consists of 530 amino acids and predictes a molecular mass of 57.4 kDa. In SDS-PAGE under reducing conditions, rh DLL1 migrates as an approximately 58 kDa band due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • The Special Role of JAK/STAT, and Notch Signaling Pathways in Cancer Pathogenesis (2023)

Gene Information

• Gene Name: DLL1 delta-like 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: DLL1
• Synonyms: DLL1; delta-like 1 (Drosophila); delta (Drosophila) like 1; delta-like protein 1; H-Delta-1; drosophila Delta homolog 1; DL1; Delta; DELTA1
• Gene ID: 28514
• mRNA Refseq: NM_005618
• Protein Refseq: NP_005609
• MIM: 606582
• UniProt ID: O00548