Active Recombinant Human DLL4 Protein, His-tagged, low Endotoxin

• Cat.No.: DLL4-11072H
• Product Overview: Human CD47 Protein, Mouse IgG2a Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3). This protein carries a mouse IgG2a Fc tag at the C-terminus. Predicted N-terminus: Gln 19

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 27-524 aa
• Description: Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human DLL4 Protein, His Tag at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human DLL4 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL.
• Molecular Mass: The protein has a calculated MW of 40.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.01 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: DLL4 delta-like 4 (Drosophila) [ Homo sapiens (human) ]
• Official Symbol: DLL4
• Synonyms: DLL4; delta-like 4 (Drosophila); delta like 4 homolog (Drosophila); delta-like protein 4; delta4; delta 4; delta ligand 4; notch ligand DLL4; delta-like 4 homolog; delta-like 4 protein; notch ligand delta-2; drosophila Delta homolog 4; hdelta2; MGC126344
• Gene ID: 54567
• mRNA Refseq: NM_019074
• Protein Refseq: NP_061947
• MIM: 605185
• UniProt ID: Q08722