Active Recombinant Human DNMT3A Protein, N-6xHis-FLAG-tagged

• Cat.No.: DNMT3A-391H
• Product Overview: Active Recombinant Human DNMT3A Protein (NP_783328.1)(214-912 aa) was expressed in Sf9 cells and contains an N-terminal 6xHis Tag and FLAG-Tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag&His
• Protein Length: 214-912 aa
• Form: Supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 1mM DTT and 0.2 mg/ml 3× Flag peptide.
• Bio-activity: Recombinant DNMT3A protein activity assay Recombinant DNMT3A protein activity was measured using DNMT Activity / Inhibition Assay, Active Motif, for 1 hour at 37°C.
• Molecular Mass: 83.8 kDa
• Purity: >95% pure by SDS-PAGE.
• Storage: ecombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_022552
• Protein Refseq: NP_072046
• MIM: 602769
• UniProt ID: Q9Y6K1