Active Recombinant Human DYRK3 protein, His&GST-tagged

• Cat.No.: DYRK3-972H
• Product Overview: Active Recombinant Human DYRK3 protein(O43781-1) (Met 1-Ser 588) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-588 aa
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% gly, pH 8.0.
• Bio-activity: The specific activity was determined to be 22 nmol/min/mg using synthetic DYRKtide peptide (RRRFRPASPLRGPPK) as substrate.
• Molecular Mass: The recombinant human DYRK3/GST chimera consists of 825 amino acids and has a calculated molecular mass of 93.5 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DYRK3 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 [ Homo sapiens ]
• Official Symbol: DYRK3
• Synonyms: DYRK3; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3; dual specificity tyrosine-phosphorylation-regulated kinase 3; dual specificity tyrosine (Y) phosphorylation regulated kinase 5; hYAK3 2; protein kinase Dyrk3; RED; REDK; regulatory erythroid kinase; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 5; DYRK5; hYAK3-2
• Gene ID: 8444
• mRNA Refseq: NM_001004023
• Protein Refseq: NP_001004023
• MIM: 603497
• UniProt ID: O43781