Active Recombinant Human EGFR protein(Met668-Ala1210), His&GST-tagged

• Cat.No.: EGFR-31H
• Product Overview: Recombinant Human EGFR (NP_005219)(Met 668-Ala 1210) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met668-Ala1210
• Form: Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
• Bio-activity: 1. The specific activity was determined to be＞70 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized EGF Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag) in functional ELISA.
• Molecular Mass: The recombinant human EGFR /GST chimera consists of 780 amino acids and has a calculated molecular mass of 89.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: Please refer to the hard copy of CoA.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533