Active Recombinant Human EIF2AK3 Protein, GST-tagged

• Cat.No.: EIF2AK3-3150H
• Product Overview: Human EIF2AK3 (NP_004827.3, 536 a.a. - 1116 a.a.) partial recombinant protein with GST tag expressed in Escherichia coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 536-1116 a.a.
• Description: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
• Form: Liquid
• Bio-activity: The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization).
  Substrate: SRPKtide.
  ATP: 100 μM.
• Molecular Mass: 94 kDa
• Purity: 51 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3 [ Homo sapiens ]
• Official Symbol: EIF2AK3
• Synonyms: EIF2AK3; eukaryotic translation initiation factor 2-alpha kinase 3; PEK; PERK; hsPEK; pancreatic EIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; eukaryotic translation initiation factor 2 alpha kinase 3; WRS; DKFZp781H1925
• Gene ID: 9451
• mRNA Refseq: NM_004836
• Protein Refseq: NP_004827
• MIM: 604032
• UniProt ID: Q9NZJ5