Active Recombinant Human ENPEP, His-tagged

• Cat.No.: ENPEP-274H
• Product Overview: Recombinant Human ENPEP(NP_001968.3) (Arg41-Gly957) was expressed with a polyhistide tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Arg41-Gly957
• Form: Supplied as sterile 20 mM, Tris 500 mM NaCl, 10 % glycerol, pH 7.4.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin (Glu-AMC). The specific activity is >2000 pmoles/min/μg.
• Molecular Mass: The recombinant human ENPEP consists of 933 amino acids and has a calculated molecular mass of 107.2 kDa. The recombinant protein migrates as an approximately 115.5 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ENPEP glutamyl aminopeptidase (aminopeptidase A) [ Homo sapiens ]
• Official Symbol: ENPEP
• Synonyms: ENPEP; glutamyl aminopeptidase (aminopeptidase A); glutamyl aminopeptidase; CD249; gp160; EAP; AP-A; aminopeptidase A; differentiation antigen gp160; APA
• Gene ID: 2028
• mRNA Refseq: NM_001977
• Protein Refseq: NP_001968
• MIM: 138297
• UniProt ID: Q07075