Active Recombinant Human ENPP7 Protein, His-tagged

• Cat.No.: ENPP7-865H
• Product Overview: Recombinant Human ENPP7 (Accession # NP_848638) Ala22-Ser439, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala22
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL and the phosphate is detected/measured by a Malachite Green Phosphate Detection Kit.The specific activity is >14,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ENPP7, His-tagged has a calculated MW of 49 kDa. In SDS-PAGE migrates as 60-70 kDa, reducing conditions.
• Protein length: Ala22-Ser439
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ENPP7 ectonucleotide pyrophosphatase/phosphodiesterase 7 [ Homo sapiens ]
• Official Symbol: ENPP7
• Synonyms: ENPP7; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; alk SMase; NPP-7; E-NPP 7; alkaline sphingomyelinase; intestinal alkaline sphingomyelinase; alkaline sphingomyelin phosphodiesterase; ALK-SMase; MGC50179
• Gene ID: 339221
• mRNA Refseq: NM_178543
• Protein Refseq: NP_848638
• UniProt ID: Q6UWV6
• Chromosome Location: 17q25.3