Active Recombinant Human ERAP1 Protein, His-tagged

• Cat.No.: ERAP1-532H
• Product Overview: Recombinant human ERAP1 (Ala37-Met941) protein with a C-terminal 10-His tag, was expressed in Mouse myeloma cell line.

Specification

• Description: The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro. Also known as ALAP (Adipocyte-derived Leucin AminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene. Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala37
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC. The specific activity is >125 pmol/min/µg
• Molecular Mass: 105 kDa
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.

Gene Information

• Gene Name: ERAP1
• Official Symbol: ERAP1 endoplasmic reticulum aminopeptidase 1 [ Homo sapiens (human) ]
• Synonyms: Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator
• Gene ID: 51752
• mRNA Refseq: NM_016442
• Protein Refseq: NP_057526
• MIM: 606832
• UniProt ID: Q9NZ08