Active Recombinant Human EREG protein, hFc-tagged

• Cat.No.: EREG-562H
• Product Overview: Active Recombinant Human EREG protein(Q61521) (Val 63-Leu 108) was expressed in HEK293, fused with the Fc region of human IgG1 at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 63-108 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-8 μg/mL.
• Molecular Mass: The recombinant human EREG/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 306 amino acids and has a predicted molecular mass of 33.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEREG/Fc monomer is approximately 37 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EREG epiregulin [ Homo sapiens ]
• Official Symbol: EREG
• Synonyms: EREG; epiregulin; proepiregulin; ER
• Gene ID: 2069
• mRNA Refseq: NM_001432
• Protein Refseq: NP_001423
• MIM: 602061
• UniProt ID: O14944