Active Recombinant Human F3 protein, His-tagged

• Cat.No.: F3-20H
• Product Overview: Recombinant Human F3(Gly34-Glu251) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 34-251 a.a.
• Predicted N Terminal: Gly34
• Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <4.0 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 26 kDa;SDS-PAGE: 41 kDa and 43 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: F3 coagulation factor III (thromboplastin, tissue factor) [ Homo sapiens ]
• Official Symbol: F3
• Synonyms: F3; coagulation factor III (thromboplastin, tissue factor); tissue factor; CD142; TF; TFA; FLJ17960
• Gene ID: 2152
• mRNA Refseq: NM_001178096
• Protein Refseq: NP_001171567
• MIM: 134390
• UniProt ID: P13726
• Chromosome Location: 1p22-p21
• Pathway: Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: cell surface binding; phospholipid binding; protease binding; protein binding