Active Recombinant Human Factor-IX
Cat.No. : | F9-4350H |
Product Overview : | Human Factor-IX produced from fresh frozen human plasma is a glycosylated polypeptide chain having a molecular mass of 56 kDa. |
- Specification
- Gene Information
- Related Products
- Download
Cat. No. : | F9-4350H |
Description : | Human Factor IX also called Christmas-Factor is a glycoprotein, which is synthesized in the liver and belongs to the serine proteases system and is part of the S1 peptidase family. Lack of Factor-IX causes Hemophilia-B meaning Christmas Disease. Factor-IX has a N-terminus region which contains 12xGla residues which asist the calcium dpendant binding of Factor-IX to the phospholipid surface. Factor-IX is activated by either factor XIa or the factor VIIa/tissue factor/phospholipid complex. Cleavage yields the intermediate IXa, which is subsequently converted to the fully active form IXab. Factor-IX binds initially to exosites on the factor XIa heavy chain, followed by interaction at the active site with subsequent bond cleavage. Coagulation factor IX is activated by interaction with the erythrocyte membrane, causing intrinsic coagulation. Chaperones & lectins act simultaniously to guarantee the proper folding of Factor-IX and the retention of mutant molecules. Human Factor IX, activated by either the Contact or Tissue Factor Pathway, is responsible for the activation of Factor X to Xa. |
Source : | Human |
Host : | Human Plasma |
Form : | The Factor-IX was lyophilized from a sterile solution containing 20mM Tris-HCl pH-7.4, 0.1M NaCl and 1mM Benzamidine. |
Purity : | Greater than 95% as determined by SDS-PAGE. |
Bio-activity : | The activity per mg was tested and found to be 195 Units per mg. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Solubility : | It is recommended to reconstitute the lyophilized 100U Factor-IX in sterile 100µl of 18Mμ-cm H2O, which can then be further diluted to other aqueous solutions. |
Storage : | Lyophilized Factor-IX although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Factor-IX should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
Pathways : | Blood Clotting Cascade; Complement and Coagulation Cascades; Extrinsic Pathway; Gamma-carboxylation of protein precursors; Hemostasis; Intrinsic Pathway; Post-translational modification: gamma carboxylation and hypusine formation; Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus |
Functions : | calcium ion binding; peptidase activity; serine-type endopeptidase activity |
Tag : | Non |
Gene Name : | F9 coagulation factor IX [ Homo sapiens ] |
Official Symbol : | FABP7 |
Synonyms : | F9; FIX; P19; PTC; HEMB; coagulation factor IX; Plasma thromboplastin component; Christmas factor; EC 3.4.21.22; factor 9; plasma thromboplastic component; EC 3.4.21; MGC129641; MGC129642 |
Gene ID : | 2158 |
mRNA Refseq : | NM_000133 |
Protein Refseq : | NP_000124 |
MIM : | 300746 |
UniProt ID : | P00740 |
Chromosome Location : | Xq27.1-q27.2 |
Products Types
◆ Recombinant Protein | ||
F9-1838R | Recombinant Rat F9 Protein, His (Fc)-Avi-tagged | +Inquiry |
F9-1358R | Recombinant Rhesus Macaque F9 Protein, His (Fc)-Avi-tagged | +Inquiry |
F9-915R | Recombinant Rabbit F9 Protein, His-tagged | +Inquiry |
F9-914R | Recombinant Rabbit F9 Protein, His-tagged | +Inquiry |
F9-1299H | Recombinant Human F9 protein, His-KSI-tagged | +Inquiry |
◆ Native Protein | ||
F9-266B | Active Native Bovine Factor IX | +Inquiry |
F9-26523TH | Native Human F9 | +Inquiry |
F9-300R | Native Rat Factor IX | +Inquiry |
◆ Lysates | ||
F9-1849HCL | Recombinant Human F9 cell lysate | +Inquiry |
F9-1768MCL | Recombinant Mouse F9 cell lysate | +Inquiry |
Related Gene
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Customer Reviews (3)
Write a reviewThe short half-life makes this product very safe.
This protein has a wide range of applications and can be used in a variety of experiments.
The method of use is simple, which reduces the experimental time and improves the efficiency.
Q&As (6)
Ask a questionThe F9 protein is involved in step IX in the coagulation cascade, which works synergistically with other coagulation factors to promote the normal progress of blood clotting.
Mutations in the F9 gene may lead to loss of protein expression, structural abnormalities, or dysfunction, which in turn affect the normal function of the F9 protein.
F9 protein mutations are primarily associated with hemophilia B, but the specific mutation type and location may have different effects on an individual's clinical presentation.
Mutations in the F9 gene may cause hemophilia B, an inherited coagulation disorder in which patients lack the F9 protein or have low activity and are prone to abnormal bleeding.
The activity of the F9 protein is usually measured by coagulation time or by specific experimental methods (eg, prothrombin time, activated partial thromboplastin time, etc.).
F9 protein is a single-chain glycoprotein consisting of 415 amino acids. It contains a signaling peptide and multiple functional regions in which the Gla domain at the C-terminal binds to calcium ions and the EGF-like domain at the N-terminus participates in receptor binding and activation.
Ask a Question for All F9 Products
Required fields are marked with *
My Review for All F9 Products
Required fields are marked with *
Inquiry Basket