Active Recombinant Human FBLN1 Protein, HA-tagged

• Cat.No.: FBLN1-01H
• Product Overview: Recombinant Human FBLN1 Protein (Asp30-Leu683) with an N-terminal HA tag was expressed in Human embryonic kidney cell, HEK293.

Specification

• Description: Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants.
• Source: HEK293
• Species: Human
• Tag: HA
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells. The ED50 for this effect is 0.7-3.5 μg/mL.
• Molecular Mass: 73 kDa
• N-terminal Sequence Analysis: Tyr
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: FBLN1 fibulin 1 [ Homo sapiens (human) ]
• Official Symbol: FBLN1
• Synonyms: FBLN1; fibulin 1; FBLN; FIBL1; fibulin-1
• Gene ID: 2192
• mRNA Refseq: NM_001996
• Protein Refseq: NP_001987
• MIM: 135820
• UniProt ID: P23142