Active Recombinant Human FCER1A protein, His-tagged

• Cat.No.: FCER1A-725H
• Product Overview: Active Recombinant Human FCER1A protein(P12319-1)(Val26-Gln205) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 26-205 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human Fc epsilon RI alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IgE, His-Avi Tag with the EC50 of 23.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FCER1A Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide [ Homo sapiens ]
• Official Symbol: FCER1A
• Synonyms: FCER1A; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCE1A; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; FcERI
• Gene ID: 2205
• mRNA Refseq: NM_002001
• Protein Refseq: NP_001992
• MIM: 147140
• UniProt ID: P12319