Active Recombinant Human FCER1A, His-tagged

• Cat.No.: FCER1A-725H
• Product Overview: Recombinant Human FCER1A (Accession # NP_001992) Val26-Gln205, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 26-205 a.a.
• Predicted N Terminal: Val26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human Fc epsilon RI alpha is immobilized at 2 µg/mL (100 µL/well), the concentration of human IgE that produces 50% of the optimal binding response is found to be approximately 0.05 - 0.2 µg/mL.
• Molecular Mass: Recombinant Human FCER1A, His-tagged has a calculated MW of 21.9 kDa. In SDS-PAGE migrates as 45-58 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: FCER1A Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide [ Homo sapiens ]
• Official Symbol: FCER1A
• Synonyms: FCER1A; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCE1A; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; FcERI
• Gene ID: 2205
• mRNA Refseq: NM_002001
• Protein Refseq: NP_001992
• MIM: 147140
• UniProt ID: P12319
• Chromosome Location: 1q23