Active Recombinant Human FGF2 Protein (Formulation I- Carrier Free-Lyophilized, 134-288, 154 amino acid)

Cat.No. : FGF2-25H
Product Overview : Recombinant Human FGF2 Protein (Formulation I- Carrier Free-Lyophilized, 134-288, 154 amino acid) without tag was expressed in E. coli.
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  • Gene Information
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Description : The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Source : E. coli
Species : Human
Form : Lyophilized
Bio-activity : ED50= 0.1-1.0 ng/mL as determined by the dose dependent proliferation of NIH 3T3 cells.
Molecular Mass : 17.3 kDa
Protein Length : 134-288, 154 amino acid
AA Sequence : AAGSITTLPALPEDGGSGAFPPGHF KDPKRLYCKNGGFFLRIHPDGRVDG VREKSDPHIKLQLQAEERGVVSIKG VCANRYLAMKEDGRLLASKCVTDEC FFFERLESNNYNTYRSRKYTSWYVA LKRTGQYKLGSKTGPGQKAILFLPM SAKS
Endotoxin : < 0.01 ng/μg cytokine as determined by the LAL assay
Purity : > 98% by SDS-PAGE
Usage : Useful for cell culture and for the study of signaling pathways.
Storage : Upon receipt, it should be stored immediately at -20 to -80 centigrade. Lyophilized bFGF is stable at -20 to -80 centigrade for up to 16 months.
Storage Buffer : Sterile filtered through a 0.22 micron filter and lyophilized from Tris buffer (10 mM Tris pH7.5, 150 mM NaCl).
Reconstitution : Centrifuge vial before opening. It is recommended to reconstitute bFGF in sterile 10 mM Tris-HCl, pH 7.5 to yield a stock solution of 0.1 mg/mL of bFGF. It is stable for up to 6 months when stored at -20 centigrade and up to 12 months when stored at -80 centigrade. Multiple freeze/thaw cycles will result in significant loss of activity.
Shipping : The product is shipped at ambient temperature with ice bags.
Gene Name : FGF2 fibroblast growth factor 2 [ Homo sapiens (human) ]
Official Symbol : FGF2
Synonyms : FGF2; fibroblast growth factor 2; BFGF; FGFB; FGF-2; HBGF-2; fibroblast growth factor 2; basic fibroblast growth factor bFGF; fibroblast growth factor 2 (basic); heparin-binding growth factor 2; prostatropin
Gene ID : 2247
mRNA Refseq : NM_002006
Protein Refseq : NP_001997
MIM : 134920
UniProt ID : P09038

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the biological function of FGF2? 11/10/2022

FGF2 promotes cell migration and angiogenesis and regulates bone homeostatic balance.

Where is FGF2 mainly expressed? 10/22/2022

FGF2 is widely expressed in the nervous system.

In which tissues of sheep is FGF2 primarily expressed? 01/01/2022

FGF2 is mainly expressed in tissues such as mammary glands and ovaries of sheep.

What protein does FGF2 share high homology with? 03/08/2021

FGF1 has a high degree of homology with FGF2.

What diseases is FGF2 involved in? 02/12/2021

FGF2 is involved in diseases such as cardiovascular disease, breast disease and bone homeostasis.

How does FGF2 regulate bone homeostasis? 11/21/2020

FGF2 negatively regulates bone homeostasis.

Customer Reviews (2)

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Reviews
07/14/2022

    The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.

    02/04/2022

      The purchased protein is suitable for WB experiments and I am satisfied with the results.

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