Active Recombinant Human FGF2 Protein (Formulation I- Carrier Free-Lyophilized, 134-288, 154 amino acid)

• Cat.No.: FGF2-25H
• Product Overview: Recombinant Human FGF2 Protein (Formulation I- Carrier Free-Lyophilized, 134-288, 154 amino acid) without tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
• Source: E. coli
• Species: Human
• Form: Lyophilized
• Bio-activity: ED50= 0.1-1.0 ng/mL as determined by the dose dependent proliferation of NIH 3T3 cells.
• Molecular Mass: 17.3 kDa
• Protein Length: 134-288, 154 amino acid
• AA Sequence: AAGSITTLPALPEDGGSGAFPPGHF KDPKRLYCKNGGFFLRIHPDGRVDG VREKSDPHIKLQLQAEERGVVSIKG VCANRYLAMKEDGRLLASKCVTDEC FFFERLESNNYNTYRSRKYTSWYVA LKRTGQYKLGSKTGPGQKAILFLPM SAKS
• Endotoxin: < 0.01 ng/μg cytokine as determined by the LAL assay
• Purity: > 98% by SDS-PAGE
• Usage: Useful for cell culture and for the study of signaling pathways.
• Storage: Upon receipt, it should be stored immediately at -20 to -80 centigrade. Lyophilized bFGF is stable at -20 to -80 centigrade for up to 16 months.
• Storage Buffer: Sterile filtered through a 0.22 micron filter and lyophilized from Tris buffer (10 mM Tris pH7.5, 150 mM NaCl).
• Reconstitution: Centrifuge vial before opening. It is recommended to reconstitute bFGF in sterile 10 mM Tris-HCl, pH 7.5 to yield a stock solution of 0.1 mg/mL of bFGF. It is stable for up to 6 months when stored at -20 centigrade and up to 12 months when stored at -80 centigrade. Multiple freeze/thaw cycles will result in significant loss of activity.
• Shipping: The product is shipped at ambient temperature with ice bags.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 [ Homo sapiens (human) ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2; BFGF; FGFB; FGF-2; HBGF-2; fibroblast growth factor 2; basic fibroblast growth factor bFGF; fibroblast growth factor 2 (basic); heparin-binding growth factor 2; prostatropin
• Gene ID: 2247
• mRNA Refseq: NM_002006
• Protein Refseq: NP_001997
• MIM: 134920
• UniProt ID: P09038

Reviews

07/14/2022

The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.

02/04/2022

The purchased protein is suitable for WB experiments and I am satisfied with the results.

Q&As

What is the biological function of FGF2?

FGF2 promotes cell migration and angiogenesis and regulates bone homeostatic balance.

Where is FGF2 mainly expressed?

FGF2 is widely expressed in the nervous system.

In which tissues of sheep is FGF2 primarily expressed?

FGF2 is mainly expressed in tissues such as mammary glands and ovaries of sheep.

What protein does FGF2 share high homology with?

FGF1 has a high degree of homology with FGF2.

What diseases is FGF2 involved in?

FGF2 is involved in diseases such as cardiovascular disease, breast disease and bone homeostasis.

How does FGF2 regulate bone homeostasis?

FGF2 negatively regulates bone homeostasis.