Active Recombinant Human FGF8 Protein

• Cat.No.: FGF8-771H
• Product Overview: Recombinant human FGF8a (NP_149355.1) (Gln23-Arg204) was expressed with an initial Met.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.5, 5% ~ 8% trehalose and mannitol.
• Bio-activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3 μg/mL.
• Molecular Mass: The recombinant human FGF8a consists 183 amino acids and predicts a molecular mass of 21.3 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Tag: Non
• Protein length: Gln23-Arg204

Gene Information

• Gene Name: FGF8 fibroblast growth factor 8 (androgen-induced) [ Homo sapiens ]
• Official Symbol: FGF8
• Synonyms: FGF8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; AIGF; androgen-induced growth factor; heparin-binding growth factor 8; KAL6; FGF-8; HBGF-8; MGC149376
• Gene ID: 2253
• mRNA Refseq: NM_001206389
• Protein Refseq: NP_001193318
• MIM: 600483
• UniProt ID: P55075
• Chromosome Location: 10q25-q26
• Pathway: Downstream signaling of activated FGFR, organism-specific biosystem; FGFR ligand binding and activation, organism-specific biosystem; FGFR1 ligand binding and activation, organism-specific biosystem; FGFR1c ligand binding and activation, organism-specific biosystem; FGFR2 ligand binding and activation, organism-specific biosystem; FGFR2c ligand binding and activation, organism-specific biosystem; FGFR3 ligand binding and activation, organism-specific biosystem

Reviews

07/08/2021

Top-quality and timely delivery. It's a pleasure working with this product.

06/30/2020

Service, quality, and shipping—all exceptional. This product is our research ally.

10/09/2019

Seamless shipping and a product that never disappoints. Kudos!

Q&As

How does FGF8 participate in embryonic development, particularly in the patterning of the midbrain and limb buds, and what molecular events coordinate its spatial and temporal expression?

FGF8 is essential for midbrain and limb bud patterning during embryonic development. Spatial and temporal expression is coordinated by signaling pathways and transcription factors that regulate FGF8 expression.

How do post-translational modifications, such as glycosylation, impact the function of FGF8, and what is the significance of these modifications in modulating ligand-receptor interactions?

Post-translational modifications, including glycosylation, can influence FGF8 function. Glycosylation may modulate ligand-receptor interactions, affecting the specificity and strength of FGF8 signaling.

What is the structural classification of FGF8, and how does its tertiary structure influence interactions with FGF receptors and heparin sulfate proteoglycans?

FGF8 is a member of the fibroblast growth factor family with a β-trefoil tertiary structure. This structure dictates its binding to FGF receptors and heparin sulfate proteoglycans, essential for signal transduction.

How does FGF8 participate in the crosstalk between different signaling pathways, such as Wnt and Shh, and what are the consequences for tissue morphogenesis and organogenesis?

FGF8 engages in crosstalk with pathways like Wnt and Shh during tissue morphogenesis. This interaction influences organogenesis by coordinating cellular processes and ensuring proper tissue patterning.

Can you discuss the interactions between FGF8 and the extracellular matrix, and how these interactions influence the stability and bioavailability of FGF8 ligands in the tissue microenvironment?

FGF8 interacts with the extracellular matrix, affecting ligand stability and bioavailability. This interaction modulates FGF8 diffusion and gradients in the tissue microenvironment.