| Description : |
Fibroblast Growth Factor Receptor-1 (FGFR-1) is a transmembrane tyrosine kinase receptor belonging to the FGFR family. FGFR family has 4 members, FGFR-1 to FGFR-4, and they all have similar structural characteristics with 3 extracellular immunoglobulin-like (Ig) domains. FGFRs bind to FGFs with the second and third Ig domains, and complex with heparin sulfate when binding. The binding to FGF induces the dimerziation of FGFR and the phosphorylations of the intracellular tyrosines. Furthermore, the phosphorylated FGFR activates downstream signaling pathways, including STAT/JAK, RAS/MAPK, and PI3 K/AKT. Particularly, the signaling of FGFR-1 is stronger than that of FGFR-2, and sustains longer than that of FGFR-4. FGFR-1 is involved in the breast cancer: the patients with the FGFR-1 amplification are more likely to develop distant metastases, and the amplification of FGFR-1 is significantly associated with a shorter overall survival. |
| Source : |
SF9 insect cells |
| Species : |
Human |
| Tag : |
Fc |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 2 ng/mL, measured by the neutralization assay using 3T3 cells in presence of 4 ng/mL of human FGF-acidic, corresponding to a specific activity of > 5 × 10^5 units/mg. |
| Molecular Mass : |
~90 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
592 |
| AA Sequence : |
RPSPTLPEQAQPWGAPVEVESFLVH PGDLLQLRCRLRDDVQSINWLRDGV QLAESNRTRITGEEVEVQDSVPADS GLYACVTSSPSGSDTTYFSVNVSDA LPSSEDDDDDDDSSSEEKETDNTKP NPVAPYWTSPEKMEKKLHAVPAAKT VKFKCPSSGTPNPTLRWLKNGKEFK PDHRIGGYKVRYATWSIIMDSVVPS DKGNYTCIVENEYGSINHTYQLDVV ERSPHRPILQAGLPANKTVALGSNV EFMCKVYSDPQPHIQWLKHIEVNGS KIGPDNLPYVQILKTAGVNTTDKEM EVLHLRNVSFEDAGEYTCLAGNSIG LSHHSAWLTVLEALEERPAVMTSPL YLEGSGSGSGSPKSCDKTHTCPPCP APELLGGPSVFLFPPKPKDTLMISR TPEVTCVVVDVSHEDPEVKFNWYVD GVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPA PIEKTISKAKGQPREPQVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVE WESNGQPENNYKTTPPVLDSDGSFF LYSKLTVDKSRWQQGNVFSCSVMHE ALHNHYTQKSLSLSPGK |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% by SDS-PAGE and HPLC analyses. |
| Storage : |
Lyophilized recombinant human FGFR-1 alpha(IIIc)-Fc (rhFGFR-1 alpha(IIIc)-Fc) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGFR-1 alpha(IIIc)-Fc remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
