Species : |
Human |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
1-328 a.a. |
Form : |
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : |
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells ( HUVEC ). ( Conn, G. et al. ,1990 , Proc. Natl. Acad. Sci. USA 87:1323. )The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165. |
Molecular Mass : |
The recombinant human FLT1 consists 313 amino acids and predicts a molecular mass of 35.6 kDa. |
Proteinlength : |
Met1-Ile328 |
Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
Purity : |
> 90 % as determined by SDS-PAGE. |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |