Recombinant Human Fms-related Tyrosine Kinase 1, 3 Domains
Cat.No. : | FLT1-78H |
Product Overview : | Recombinant Human Soluble Fms-related Tyrosine Kinase 1 D1-3 produced in baculovirus is monomeric, glycosylated, polypeptide containing 352 amino acids and having a molecular mass of 45 kDa. The soluble receptor protein contains only the first 3 extracellular domains, which contain all the information necessary for binding of VEGF. The VEGFR1 is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
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Cat. No. : | FLT1-78H |
Description : | Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor. |
Source : | Insect Cells |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | VEGFR-1 D1-3 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. |
Solubility : | It is recommended to reconstitute the lyophilized sVEGFR-1 D3 in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity : | The activity of sVEGFR-1D1-3 was determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surfaces receptors, and in Far-Western and cross-linking experiments with iodinated VEGF. |
Storage : | Lyophilized FLT-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FLT1 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Gene Name : | FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) [ Homo sapiens ] |
Synonyms : | FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; VEGFR1; vascular endothelial growth factor receptor 1; EC 2.7.10.1; VEGFR-1; Vascular permeability factor receptor; Flt-1; Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-like tyrosine kinase 1; FRT |
Gene ID : | 2321 |
mRNA Refseq : | NM_001159920 |
Protein Refseq : | NP_001153392 |
MIM : | 165070 |
UniProt ID : | P17948 |
Chromosome Location : | 13q12 |
Pathway : | Cytokine-cytokine receptor interaction; Endocytosis; Focal adhesion; Signaling by VEGF |
Function : | ATP binding; growth factor binding; identical protein binding; nucleotide binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity |
Products Types
◆ Recombinant Protein | ||
FLT1-372H | Recombinant Human FLT1 Protein, His-tagged | +Inquiry |
Flt1-3044M | Recombinant Mouse Flt1 Protein, Myc/DDK-tagged | +Inquiry |
Flt1-49M | Recombinant Mouse Flt1 Protein, His (Fc)-Avi-tagged | +Inquiry |
FLT1-95H | Recombinant Human FLT1 Protein (ECD), 8X His-tagged(C-ter) | +Inquiry |
FLT1-1314H | Recombinant Human FLT1 Protein (P801-L1158), His tagged | +Inquiry |
◆ Lysates | ||
FLT1-1207RCL | Recombinant Rat FLT1 cell lysate | +Inquiry |
FLT1-1909HCL | Recombinant Human FLT1 cell lysate | +Inquiry |
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