Active Recombinant Human FLT1 protein, His-tagged

• Cat.No.: FLT1-530H
• Product Overview: Recombinant Human FLT1(NP_002010.2) (Met1-Ile328) fused with His tag was produced in HEK293F cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ile328
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). (Conn, G. et al. ,1990 , Proc. Natl. Acad. Sci. USA 87:1323.)
  The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165.
• Molecular Mass: The recombinant human FLT1 consists 313 amino acids and predicts a molecular mass of 35.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) [ Homo sapiens ]
• Official Symbol: FLT1
• Synonyms: FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; vascular endothelial growth factor receptor 1; VEGFR1; FLT-1; VEGFR-1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor
• Gene ID: 2321
• mRNA Refseq: NM_001159920
• Protein Refseq: NP_001153392
• MIM: 165070
• UniProt ID: P17948