Active Recombinant Human Flt1 protein, His-tagged

• Cat.No.: Flt1-0826H
• Product Overview: Active Recombinant Human Flt1 protein(Accession #NP_002010.1)(Met1-Asn756) is expressed in HEK293, fused with a 8xHis tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-756 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGFR1 at 500 ng/mL (100 μL/well) can bind Recombinant Mouse PLGF with a linear range of 12-49 ng/mL. 2.Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.039-0.154 ng/mL.
• Molecular Mass: 85.69 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) [ Homo sapiens ]
• Official Symbol: Flt1
• Synonyms: FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; vascular endothelial growth factor receptor 1; VEGFR1; FLT-1; VEGFR-1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor
• Gene ID: 2321
• mRNA Refseq: NM_001159920
• Protein Refseq: NP_001153392
• MIM: 165070
• UniProt ID: P17948