Active Recombinant Human FLT3 (D835Y), GST-tagged

• Cat.No.: FLT3-259H
• Product Overview: Recombinant human FLT3 (D835Y) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: FLT3 is a receptor tyrosine kinase that has been shown to play a role in proliferation and survival of hematopoietic progenitor cells as well as differentiation of early B lymphoid progenitors. FLT3 consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. FLT3 is the most frequently mutated gene in cases of acute myelogenous leukemia (AML). About 30% to 35% of patients have either internal tandem duplications (ITDs) in the juxtamembrane domain or mutations in the activating loop of FLT3. The consequence of either FLT3-ITD or activating loop mutations is the constitutive activation of the tyrosine kinase activity.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of FLT3 (D835Y) was determined to be 46 nmol /min/mg
• Molecular Mass: ~73 kDa
• Purity: >70% by densitometry
• Applications: Kinase Assay, Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 μg/ml
• Protein length: 571-993

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Official Symbol: FLT3
• Synonyms: FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III; FLK-2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888
• Chromosome Location: 13q12
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: ATP binding; cytokine receptor activity; nucleotide binding; phosphatidylinositol 3-kinase binding; protein homodimerization activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor-activated receptor activity

Reviews

08/01/2021

Excellent product, great price.

12/22/2020

Satisfied, will order again.

09/14/2020

Quick response, helpful service.

Q&As

How is FLT3 implicated in acute myeloid leukemia (AML)?

FLT3 is frequently mutated in AML, contributing to uncontrolled cell growth and poor prognosis.

In which cells or tissues is FLT3 predominantly expressed?

FLT3 is predominantly expressed in hematopoietic stem cells and progenitor cells.

How does FLT3 contribute to hematopoiesis?

It is essential for hematopoiesis, particularly in the proliferation of early progenitor cells.

Are there therapeutic strategies targeting FLT3 for disease treatment?

Therapeutic strategies include FLT3 inhibitors, showing effectiveness in treating AML patients with FLT3 mutations.

What is the primary biological function of FLT3 in cells?

FLT3 is a receptor tyrosine kinase that plays a crucial role in cell survival, proliferation, and differentiation.

What role does FLT3 play in the proliferation and differentiation of hematopoietic stem cells?

It stimulates the proliferation and differentiation of hematopoietic stem cells, guiding their development into various blood cell types.

What are the common mutations found in FLT3 associated with diseases?

Common mutations include internal tandem duplications (ITD) and tyrosine kinase domain (TKD) mutations, leading to constitutive activation of FLT3.