Active Recombinant Human FOLR1 protein, His-tagged

• Cat.No.: FOLR1-030H
• Product Overview: Recombinant Human FOLR1 protein(Arg 25 - Met 233), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Arg25-Met233
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 . Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Folic Acid-BSA Conjugate at 5 μg/mL (100 μL/well) can bind Human FOLR1, His Tag with a linear range of 5-78 ng/mL.Immobilized Human FOLR1, His Tag at 1 μg/mL (100 μL/well) can bind Anti-FOLR1 Antibody,Mouse IgG1 with a linear range of 0.2-1 ng/mL.Immobilized Anti-FOLR1 Antibody,Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human FOLR1, His Tag with a linear range of 1-5 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-40 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FOLR1 folate receptor 1 (adult) [ Homo sapiens ]
• Official Symbol: FOLR1
• Synonyms: FOLR1; folate receptor 1 (adult); FOLR; folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18; FBP
• Gene ID: 2348
• mRNA Refseq: NM_000802
• Protein Refseq: NP_000793
• MIM: 136430
• UniProt ID: P15328